Cholesterol-Dependent Cellular Processes and Peptides Containing Cholesterol-Binding Motifs: Possible Implications for Medicine

Cholesterol-Binding Peptides and Phagocytosis

A Possible Mechanism of Cellular Cholesterol Efflux*

After in vitro incubation with radiolabeled amino acids, extracts of various chicken tissues were reacted with antiserum against apolipoprotein AI (apo-AI) of plasma high density lipoprotein. Radiolabeled apo-AI was found in liver and intestine as well as in the kidney and a variety of peripheral tissues including aorta, peripheral arteries, veins, and skeletal muscle. The immunoreactive apo-AI...

Binding of amphiphilic peptides to phospholipid/cholesterol unilamellar vesicles: a model for protein--cholesterol interaction.

In earlier studies, we prepared a docosapeptide, 1, designed with minimum homology as an amphiphilic alpha-helical model of apolipoprotein A-I (apo A-I) and described its lipid-binding characteristics, surface properties, and enzyme-activating ability. Although the affinity of 1 for egg lecithin unilamellar vesicles was comparable with that for the binding of apo A-I, the affinity of 1 for mixe...

Consequences of cellular cholesterol accumulation: basic concepts and physiological implications.

cells of most organs and tissues satisfy their requirements for membrane cholesterol via endogenous cholesterol biosynthesis (1). Many cell types, however, have acquired mechanisms to internalize exogenous sources of cholesterol, usually in the form of plasma-derived lipoproteins (2). Examples include steroid-synthesizing cells, hepatocytes, and macrophages and smooth muscle cells in atheroscle...

high cholesterol diet increases expression of cholesterol 24-hydroxylase and bace1 in rat hippocampi: implications for the effect of diet cholesterol on memory

background abnormal cholesterol homeostasis is associated with the pathogenesis of neurodegenerative disease and cognitive impairment. objectives our objective was to evaluate changes in the expression of proteins related to cognition and cholesterol homeostasis in the hippocampi of rats as well as behavioral modifications following the administration of a cholesterol-rich diet. methods in this...